Assessing the Potential for 3D Printed Equipment in Cleanrooms

a) Wafer box for four 100 mm wafer quarters 3-D printed out of PLA including top (black), bottom (blue) and holder (black ring). b) Open SCAD model of the customizable single wafer box. c) Wafer quarters stored securely in the 3-D printed box. d) Wafer quarters free to move in a commercial single wafer box.

3D printing has been used by scientists to save money on lab equipment, which is typically quite expensive. Things get a bit complicated, however, when it comes to equipment that is used in clean rooms. There are strict limitations on the types of materials and items that are allowed in cleanrooms, so a good deal of study and experimentation must be done before clearing a new item or material for use. That is the purpose of a study entitled “Compatibility of 3-D Printed Devices in Cleanroom Environments for Semiconductor Processing.”

“As the dimensions of typical semiconductor devices are in the micrometer range, it is essential to fabricate those components in an environment, where the level of contaminants (e.g. dust particles and organic compounds) is accurately controlled,” the researchers explain. “In cleanrooms, the level of contamination is specified by the number of particles per cubic meter at specified particle sizes by the international ISO (the International Organization for Standardization) standards.”

To meet these requirements, air flowing into the cleanroom is filtered and recirculated through HEPA filters, and operators wear protective clothing. Limitations are set on the materials that can be used to make cleanroom equipment and tools, such as wafer boxes and tweezers, since they are only allowed to generate a minimal amount of particles.

“The use of FFF-based 3-D printing in the cleanroom is limited because of the particles generated during fabrication itself, which depend on numerous factors including filament type, filament color, printing parameters and printer design,” the researchers continue.

The study takes a look at the possibility of using 3D printing for some of the least strenuous applications in the cleanroom environment – those that do not require chemical compatibility. The researchers used two objects – a custom single wafer storage box and a wafer positioner for a metrology system – and tested three 3D printing materials: ABS, PLA and PP, 3D printing them on a LulzBot TAZ 6 3D printer.

Increase in particle count during 15 days storage in various single wafer boxes. The initial number of particles on all wafers were in the order of 10-20. The dashed lines indicate reference levels set by the commercial PP box, which is commonly used in cleanroom environments. The error bars are determined from the variation in several repeated measurements.

The results of the study show that single wafer boxes 3D printed from PLA and ABS generate as few particles as a commercial equivalent, while slightly more particles were found in the PP box.

“The 3-D wafer positioner seems to cause a negligible particle increase on the manipulated wafer, while abrasion of the mechanical parts generate larger numbers of particles that may disperse in the environment,” the researchers state. “Regular cleaning of those parts is thus recommended, and applicability in a cleanroom environment will depend on the cleanliness constraints.”

Elemental analysis showed that 3D printed objects contained no harmful metal impurities, other than those resulting from colorants, so the researchers recommend that only natural-colored filament be used, especially in applications where metal contamination could be an issue, such as in semiconductor processing.

a) Parts of the positioner modelled in the OpenSCAD software. b) 3-D printed and assembled positioner.

The filaments studied also showed themselves to be resistant to isopropanol and deionized water, which are used for the cleaning of objects in cleanrooms. The researchers conclude that 3D printing is a safe method of creating objects for use in cleanrooms, enabling scientists to take advantage of the cost savings that the technology offers.

Authors of the paper include T.P. Pasanen, G. von Gastrow, I.T.S. Heikkinen, V. Vähänissi, H. Savin and J.M. Pearce.

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3D Printing Inexpensive Lab Equipment with the Custom Lab Institute

One thing we’re particularly passionate about at 3DPrint.com is when 3D printing is an enabler. When our technology is used to radically change others lives, industries and practices through being a force multiplier for them. In custom prosthetics, we can see huge impacts in people’s lives because 3D printing can make tough inexpensive prosthetics. This is still one of the most inspiring examples so far of the desktop side of our industry making an impact. Another thing that could operate in a similar manner is for 3D printing to be harnassed for lab equipment and scientific research. Lab equipment and all manner of scientific tools have been hampered by high costs and the fact that many things are low volume and may even be unique. Jars, testing vessels, tests, holders, housings and the like can cost four or forty times more than a comparable item in another industry. This slows down science by making it needlessly expensive. This means that we as a world are learning less quickly than we could because of a few companies making outsized margins in niches. This is a particular problem that 3D printing can help solve in many areas and we will see this happening again and again.

The Custom-Lab Institute has just gotten started but it and many initiatives like it could really bring down the cost of scientific testing. The custom lab uses stereolithography to make lab equipment for less. Whats more, this equipment can be customized to the user’s needs. We interviewed Uli Lutz who is a Biologist at the Max Planck Institute for Developmental Biology and founded the Custom Lab.

Why are researchers 3D printing their own lab equipment?

Researchers started to print lab equipment for several reasons. I think the main motivations are the reproduction and optimization of equipment, which is already available on the market but hopelessly overpriced and not fitting specific needs. Further, researchers often deal with highly specific problems, which require highly specific tools not available on the market. These tools often are surpisingly simple, but help to save a lot of time.

What kind of 3D printed lab equipment is there?

The spectrum of 3D printed lab equipment got quite broad in the last few years. On the one hand, there are simple but helpful tools like reaction tube racks, which fit specific needs (e.g. tube number, or in which angle the tube is kept). On the other hand, 3D printing helps researchers to develop more complicated instruments like for example microscopes. Its kind of an irony that scientists are always all about sharing information and yet they for free supply publishers with articles and these then get locked away behind closed doors.

Open Lab Equipment, from the Custom Lab.

Open Lab Equipment, from the Custom Lab.

Is the open part of the equation important to you?

Yes, it’s a fundamental part. The whole project is inspired by the open-science-hardware movement.

Why do you use SLA?

We decided to start with SLA because we liked the fine structure, high precision and the stability of the prints, all being important for the project I had in mind when started with 3D printing in the lab. For sure, other technologies might become more interesting for future projects!

Don’t you worry that test samples may react with the SLA resins?

No, that’s not an issue, because all tools are used in combination with special reaction tubes. The samples never get in contact with any part of the 3D printed tools.

3D Printed Lab Equipment

3D Printed Lab Equipment

Is this a business for you?

No. It’s rather an experiment, to figure out whether the spectrum of users of open science hardware can be expanded by offering the tools ready-to-use, to thereby circumvent the still limited accessibility of 3D printing hardware for researchers.

What kind of things do you see researchers print in the future?

All kinds of. Better accessibility, easier usability and newer technologies of 3D printing will help researchers to realize even more specific ideas. I hope that very soon every institute or university department will have a 3D printer to print own designs and to reproduce designs deposited at repositories by colleagues.

We applaud this initiative and can’t wait to see more 3D printed science the world over. Check out the Custom-Lab Institute here.

Using 3D Printing to Sample the Ocean Floor

When we think of biodiversity, we may think of forests with wildly differing species of birds, insects and other animals, or seas with wide varieties of fish. Sometimes biodiversity is easily visible in these larger species, but often it can only be measured on a very small scale. Dr. Matthew Cannon, a research associate in the lab of Dr. David Serre at the University of Maryland School of Medicine’s Institute for Genome Sciences, is interested in measuring biodiversity using DNA from environmental samples such as fresh or marine water, sediments or soils.

The analysis of environmental DNA, or eDNA, is an effective technique of measuring biodiversity. Organisms living in a particular area can be identified and characterized by the cells and hair they leave behind, or their decaying remains, all of which contain DNA and can reveal to scientists the types of creatures that are present in any given location. Special tools are required for this kind of analysis, especially for the type of work that Dr. Cannon wants to do, which involves taking samples from deep underwater locations.

Methods of sampling eDNA from deep underwater locations are limited by the volume of water that can be collected, or because of potential contamination from surface water. The possibilities presented by collection of eDNA from these deep-water locations are intriguing, however, because a single sample can give researchers an idea of the total biodiversity of a site without direct organism sampling. These locations are difficult to explore; traditional methods such as collecting samples in trawl nets or expeditions with remotely operated vehicles are expensive and can miss organisms that can’t be captured by a net or that avoid the lights of a rover.

Therefore, Dr. Cannon wanted to explore alternative options for deep-water eDNA sampling. He designed and 3D printed a device that houses a water filter and pump, controlled by an Arduino, that can collect samples at any depth. The device allows for the collection of large samples, limited only by filtering time.

“3-D printing is allowing us to develop a prototype water sampler that might not have been practical to imagine or design a few years ago,” Dr. Cannon said.

Dr. Cannon used the 3D printer at the Health Sciences/Human Services Library Innovation Space to create his prototype, which he is now testing to ensure that the parts work well together. It only takes a few hours to 3D print each prototype, allowing him to quickly develop new iterations.

The University of Maryland prioritizes technological advancement; towards the end of last year the university opened a new center dedicated to bioengineering, and was one of the earlier schools to open a MakerBot Innovation Center. The school is responsible for some advanced 3D printing-related research, and Dr. Cannon’s work will put the university on the map once again for its use of technology to gain new insight into areas that have previously been unexplored.

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[Source/Images: University of Maryland]